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Exposing the lies and seeking truth doesn't = Hate. This is not a Kate Hate Site. A 'Kate Hate Site" wouldn't have "Shame on Kate AND Shame on Jon" in the reaction box.

Just a place where bloggers and commenters can share an open discussion on all things related to the Gosselins.

This blog was created to expose the spins and lies shared by the media and by several individuals who continue to spread filth and garbage for their own self-interests.

This blog will expose those who continue to spread lies as "truth" and those who base their beliefs and support for one individual based on those lies.
Pursuing Truth, Exposing Lies and Informing the Sheeple

July 30, 2012

Researchers have revived the Ebola Virus using Cloned DNA

Interesting. Back in 2007, researchers have boasted how they revive the deadly and contagious Ebola virus using DNA.

Fast forward to 2012 and guess what? There is an Ebola Virus breakout in Ughanda.

Source: BBC

"Fourteen people have now died since the outbreak began in western Uganda three weeks ago, he said in a broadcast."

Reverse Genetics Approach for Generating
Ebola Virus and Other Filoviruses from
Cloned DNA


Patent No: US 7,211,378 B2
Date: May 1st, 2007

OVERVIEW
Ebola virus, a negative strand RNA virus in the family Filoviridae, is among the most lethal
human pathogens. Efforts to develop treatments for Ebola infections have been hampered
by a lack of effective ways to experimentally mutate the virus

THE INVENTION
Kawaoka and his colleagues have now developed a reverse genetics approach for generating Ebola virus entirely from cloned cDNA. They prepared the full Ebola genome through reverse transcription of viral RNA, followed by PCR amplification and cloning of Ebola cDNA. They then successfully produced infectious viral particles by transfecting host cells with plasmids carrying Ebola cDNA, along with plasmids expressing Ebola proteins L, NP, VP30 and VP35 (needed for transcription and replication of negative strand RNA viruses), and one encoding the T7 RNA polymerase.

The researchers also used the system to make mutant virus particles containing an altered furin recognition motif. Furin cleaves Ebola virus glycoprotein at a highly conserved sequence motif, an event hypothesized to be critical to viral pathogenicity. However, viral
particles carrying the altered motif still showed pathogenicity and ability to replicate in culture. This result illustrates the system’s utility for hastening our understanding of the Ebola virus life cycle and the development of anti-viral agents.

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4 comments:

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